Blame it on Southern, but it's a western blot

Understanding the Western Blot

Inquiries about various issues relating to Western blot (WB) testing are frequently posted to the Lyme disease discussion groups on the Internet. Among the most commonly asked questions are: What laboratory techniques are used to carry out the assay? What exactly is being measured? What is a “band”? How are the results interpreted? What are the CDC criteria for a “positive” test? Although some ...

Bulging glands? Blame it on B cells.

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The fastest Western in town: a contemporary twist on the classic Western blot analysis.

The Western blot techniques that were originally established in the late 1970s are still actively utilized today. However, this traditional method of Western blotting has several drawbacks that include low quality resolution, spurious bands, decreased sensitivity, and poor protein integrity. Recent advances have drastically improved numerous aspects of the standard Western blot protocol to prod...

The fallibility of HIV western blot.

Western blot profile in HIV infection.

BACKGROUND Although the overall sensitivity and specificity of the western blot (WB) test for detection of antibodies to various viral proteins is high, there has been a substantial difference in the timing of the appearance of antibody bands and their intensities during different stages of HIV infection. AIMS Mapping different band patterns of Western blot results and correlating them with s...